[No.RCDN001N] StemRNA Neuro (3 x 10^6 cells)

Wild-type human brain cell neurons for general purpose assays

Formerly called ReproNeuro.

Frozen human iPSC-derived neurons for 96-well high throughput and high content assays

• Frozen as a suspension of individualized neuroprogenitor cells
• Rapidly differentiate into neurons during in vitro growth and maturation
• Functional for in vitro neurotoxicity assays and drug discovery
• Form dendritic connections leading to synchronized burst behavior around week 6 in vitro
• Responsive to various reference compounds that modulate neuron electrophysiology
• Expresses multiple neuron-specific markers, indicating a mixed population of neuron types
• Two different culture media are available separately

StemRNA Neuro iPS-derived neurons are cryopreserved using REPROCELL’s proprietary cryopreservation technology. Thawed and plated StemRNA Neuro cells mature during 2-3 weeks of culture on surfaces treated with Neuro Coat (RCDN201).

Types of ReproNeuro Media

Note that one of the following media is required for proper maturation of the StemRNA Neuro cells:

• Neuro Culture Medium (RCDN101); standard medium for general purpose assays
• Neuro MQ Culture Medium (RCDN102); a specialty medium formulated with rat astrocyte supplementation resulting in maximum performance in MEA (multiple electrode array) assays

Types of StemRNA Neuro Cells

Two different versions of StemRNA Neuro cells are currently available. Both are nearly functionally equivalent but differ in how they were derived. If you have a preference, please specify one in your order.

• Lot “A” is from an iPS cell derived by retrovirus technology and from genetic background A. This lot is the isogenic control for StemRNA Neuro AD-Mutation cells (RCDN002N).
• Lot “B” is from an iPS cell derived by RNA-reprogramming technology and is from genetic background B (32 yr old healthy male)

Intracellular Ca2+signaling imagine

The dynamic modulation of Ca²⁺concentration in StemRNA Neuro differentiated neuronal cells can be observed with the application of a fluorescent calcium binding dye (Fluo-8). Glutamine-induced calcium flux can be observed in nearly all of the visible neurons.

Before induction

After 100 uM Glutamine induction

Dopamine release by dopaminergic neurons derived from ReproNeuro cells

StemRNA Neuro cells derived from human iPS cells were first treated with high concentration Ca2+(56 uM) to polarize the cells. After polarization, increased dopamine concentration in the culture medium was detected, indicating the functional capability of dopamine release in StemRNA Neuro cells.

Dopamine uptake by dopaminergic neurons derived from StemRNA Neuro cells

StemRNA Neuro cells derived from human iPS cells were treated with DMSO or DAT (Dopamine Transporter) inhibitor (GBR12909, 20 uM). Dopamine uptake was measured by Neurotransmitter transporter uptake assay kit (Molecular Devices). The fluorescence intensity of controls decreased after addition of DAT inhibitor, indicating a block of neurotransmitter transporter in ReproNeuro.

More information: reprocell.com