[No.04-0022] Cyclopamine

SPECIFICATION

Product Name: Stemolecule Cyclopamine

Catalog Number: 04-0022

Size: 2 mg

Alternate Name(s): (3S,3′R,3’aS,6′S,6aS,6bS,7’aR,9R,11aS,11bR)-3′,6′,10,11b tetramethylspiro[2,3,4,6,6a,6b,7,8,11,11a-decahydro-1H-benzo[a]fluorene-9,2′-3a,4,5,6,7,7a-hexahydro-3H-furo[3,2-b]pyridine]-3-ol

Chemical Formula: C₂₇H₄₁NO₂

Molecular Weight: 411.62

CAS Number: 4449-51-8

Purity: Greater than 99% by TLC analysis

Formulation: White crystalline solid or clear solid film at bottom of vial

Solubility: For a 10 mM concentrated stock solution of Cyclopamine, reconstitute the compound by adding 485.9 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is reported to be soluble in DMSO at 100 mM, ethanol at 49 mM, and methanol at 17 mM.

Storage and Stability: Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C. The effect of storage of stock solutions should be verified for each application.

Quality Control: The purity of Cyclopamine was determined by TLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Cyclopamine was tested on mouse embryonic stem cells.