ICC analysis of SSEA-4 Antibody on H1 human ES cells at 10X magnification. [A] Phase contrast view of hES cell colony in culture, [B] same field of view after staining with StainAlive SSEA-1 Antibody (DyLight 550) using a 1:100 dilution.
Stage-specific embryonic antigen-4 (SSEA-4) is expressed in a variety of human cell lines including embryonic carcinoma (EC), embryonic stem (ES), and induced pluripotent stem (iPS) cells. SSEA-4 is down-regulated upon differentiation, making it a widely used marker to characterize human ES cells and monitor their differentiation. In mouse, SSEA-4 is expressed on oocytes and early cleavage-stage embryos, but not on EC or ES cells. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide. ES cell morphology, proliferation, and expression of pluripotency markers does not change after staining with this antibody.