[No.03-0001] Stemfactor Activin A, Human Recombinant (5ug)
Stemfactor Activin A is a crystallography-grade preparation providing the highest purity available. Activin A is bi-modal in action having been shown to maintain pluripotency of stem cells and promote differentiation1,2,3,4. Mature recombinant human Activin A is a ~25 kDa disulfide-linked homodimer of two 116 amino acid residue βA subunits.
Stemgent conveniently provides all the components comprising the 5i/L/A media supplement necessary to support naïve pluripotent stem cell applications.
5i (inhibitors):
- Stemolecule PD0325901 (04-0006),
- Stemolecule Y27632 (04-0012),
- Stemolecule WH-4-023 (04-0079),
- Stemolecule SB590885 (04-0080),
- Stemolecule IM-12 (04-0081) or alternatively, Stemolecule CHIR99021 (04-0004)
L: Stemfactor LIF, Human Recombinant (03-0016), and
A: Stemfactor Activin A, Human Recombinant (03-0001)
More information: reprocell.com
SPECIFICATION
Product Name
Stemfactor Activin A, Human Recombinant
Catalog Number
03-0001
Size
5 µg
Purity
Greater than 98% by SDS-PAGE analysis
Formulation
Lyophilized from 20% acetonitrile
Reconstitution
Centrifuge briefly and reconstitute Activin A in 10 mM HCl before dilution in cell culture medium. Avoid freeze-thaw cycles as it can result in loss of activity.
Storage and Stability
Activin A is shipped at room temperature. Lyophilized Activin A is stable for up to 6 months from date of receipt when stored at −20 °C to −80 °C. Reconstituted Activin A, at concentrations greater than 0.1 mg/mL, is stable for up to 3 months when stored at −20 °C and for 1 year when stored at −80 °C.
Source
E. coli
Amino Acid Sequence
GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS
Uniprot Accession Number
P08476, residues 311-426
Endotoxin Level
Less than 1.0 EU/µg of Activin A as determined by the LAL method.
Biologic Activity
The ED50 is less than 6 ng/ml as determined by its ability to inhibit the proliferation of mouse MPC-11 cells.
RESOURCE
Specification Sheets
Safety Data Sheets
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