Stemfactor Activin A, Human Recombinant
Greater than 98% by SDS-PAGE analysis
Lyophilized from 20% acetonitrile
Centrifuge briefly and reconstitute Activin A in 10 mM HCl before dilution in cell culture medium. Avoid freeze-thaw cycles as it can result in loss of activity.
Storage and Stability
Activin A is shipped at room temperature. Lyophilized Activin A is stable for up to 6 months from date of receipt when stored at −20 °C to −80 °C. Reconstituted Activin A, at concentrations greater than 0.1 mg/mL, is stable for up to 3 months when stored at −20 °C and for 1 year when stored at −80 °C.
Amino Acid Sequence
GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS
Uniprot Accession Number
P08476, residues 311-426
Less than 1.0 EU/µg of Activin A as determined by the LAL method.
The ED50 is less than 6 ng/ml as determined by its ability to inhibit the proliferation of mouse MPC-11 cells.