Stemfactor BMP-4, Human Recombinant
Greater than 95% by SDS-PAGE analysis.
Lyophilized from sterile-filtered 1 M NaCl, 50 mM NaOAc, pH 4.5.
Centrifuge briefly and then reconstitute BMP-4 in 4 mM HCl to yield a stock solution of no less than 0.1 mg/mL. Avoid freeze-thaw cycles as they can result in loss of activity.
Storage and Stability
Stemfactor BMP-4 is shipped at room temperature. Lyophilized BMP-4 is stable for up to 6 months from date of receipt when stored at −20 °C to −80 °C. Reconstituted BMP-4, at concentrations greater than or equal to 0.1 mg/mL, is stable for up to 3 months when stored at −20 °C and up to 6 months when stored at −80 °C.
Tested to be negative forMycoplasmasp. by PCR and microbial contamination by a sterility test.
Stemfactor BMP-4 was expressed in and purified from human 293 cells.
Amino Acid Sequence
SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR
Uniprot Accession Number
P12644, residues 293-408
Less than 1.0 EU/µg of BMP-4 as determined by the LAL method.
The ED50 is less than 30 ng/mL as determined by its ability to induce alkaline phosphatase production by mouse chondrogenic ATDC-5 cells.