NutriStem hPSC XF Medium (formerly called NutriStem XF/FF Culture Medium) is a fully defined xeno-free, low growth factor human embryonic stem (ES) and induced pluripotent stem (iPS) cell feeder-free culture medium that enables maintenance and expansion of pluripotent stem cells. NutriStem Medium can be used to culture pluripotent stem cells for at least 20 passages while retaining pluripotency marker expression, robust proliferation with a normal karyotype, and the ability to differentiate into cells of all three germ layers in vitro and in vivo. NutriStem Medium offers the ability to culture cells in a completely xeno-free medium without the need for high levels of basic FGF and other stimulatory growth factors and cytokines. In addition, the superior cell attachment and proliferation observed with NutriStem Medium aid high-throughput screening applications.
Xeno-free, feeder-free conditions contain no animal components
Low basic FGF (4 ng/mL), and TGFβ
Easy, one-step transition from feeder-dependent culture, no adaptation required
Maintains pluripotency, normal morphology, karyotype, and differentiation potential of human ES and iPS cells over long term culture
Robust attachment and high cloning efficiency from single cells
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