[No.03-0002] Stemfactor FGF-basic, Human Recombinant (50ug)

SPECIFICATION

Product Name

Stemfactor FGF-basic, Human Recombinant

Catalog Number

03-0002

Size

50 µg

Purity

Greater than 95% by SDS-PAGE analysis

Formulation

Lyophilized from 5 mM Tris-HCl, pH 7.5 with 150 mM NaCl.

Reconstitution

Centrifuge briefly and then reconstitute bFGF in 500 µL of 10 mM Tris, pH 7.6, to yield a stock solution of 0.1 mg/mL of bFGF. Avoid freeze-thaw cycles as it can result in loss of activity.

Storage and Stability

Stemfactor bFGF is shipped at room temperature. Lyophilized bFGF is stable for up to 6 months from date of receipt when stored at −20 °C to −80 °C. Reconstituted bFGF, at concentrations greater than or equal to 0.1 mg/mL, is stable for up to 3 months when stored at −20 °C and up to 6 months when stored at −80 °C.

Sterility

Tested to be negative forMycoplasmasp. by PCR and microbial contamination by a sterility test.

Source

E. coli

Amino Acid Sequence

MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS

Uniprot Accession Number

P09038, residues 1-155

Endotoxin Level

Less than 1.0 EU/µg of bFGF as determined by the LAL method.

Biologic Activity

The ED50 is 0.1 to 1 ng/mL as determined by the dose dependent proliferation of NIH 3T3 cells.