[No.00-0076] StemRNA 3rd Gen Reprogramming Kit
Third Generation RNA Kit for Cellular Reprogramming of Fibroblasts, Blood, and Urine
The latest evolution in Stemgent RNA reprogramming, the StemRNA 3rd Gen Reprogramming Kit (formerly called the StemRNA-NM Reprogramming Kit), combines non-modified RNA and microRNA technology to provide a kit for stem cell researchers with a new level of versatility, simplicity and time savings, enabling cellular reprogramming of human fibroblasts, and cells from blood and now urine for difficult to reprogram patient samples.
More information: reprocell.com
Key Benefits
- Flexible technology generates high-quality human iPS cell lines from multiple target cell types
Out-of-the-box reprogramming of cells from skin (fibroblasts), blood (endothelial progenitor cells; EPCs) and urine (urine-derived progenitor cells; UPCs) - High efficiency, non-integrating reprogramming
StemRNA-3rd Gen requires a few as four additional reagents. Double stranded microRNAs enhance reprogramming efficiency, providing iPS cells with high efficiency (up to 4% from fibroblasts, up to 3% from EPCs, up to 0.5% from UPCs). - Time-saving protocol delivers faster results facilitating higher throughput
Colonies ready to pick in 10-14 days from fibroblasts and 12-16 days from EPCs or UPCs. No screening needed
| StemRNA-3rd Gen Reprogramming Kit (00-0076) | |||
|---|---|---|---|
| Feature | Fibroblasts | Urine (UPCs) | Blood (EPCs) |
| No. wells per kit | 9 | 3 | 3 |
| No Transfections required | 4 | 6-8 | 6-8 |
| Days to primary iPS cell colonies | 10-14 | 12-14 | 12-14 |
| Reprogramming efficiency | 2-+4% | 0.1-0.5% | 0.4-3% |
| Screening Required | No | No | No |
| Xeno-compatible protocol | Yes | No | No |
| GMP-compatible RNA manufacturing protocol | Yes | Yes | Yes |
P7 primary iPS cells derived from UPCs were stained using the StainAlive TRA-1-60 Antibody (Cat. No. 09‑0068) and DAPI after expansion in NutriStem hPSC XF Medium (Cat. No. 01‑0005)
iPS cells derived from fibroblasts using the StemRNA-3rd Gen Reprogramming Kit were cultured on iMatrix-511 (Cat. No. NP892‑011) in NutriStem hPSC XF Medium (Cat. No. 01‑0005) for 7 passages. Magnification: 4x
Timeline for StemRNA 3rd reprogramming of fibroblasts
| Product Name | StemRNA 3rd Gen Reprogramming Kit |
|---|---|
| Catalog Number | 00-0076 |
| Storage and Stability | Store all three kit components at or below −70 °C. Kit components are stable for a minimum of 3 months from date of receipt when stored as directed. |
| Quality Control | The individual mRNAs are tested for size and integrity. The StemRNA 3rd Gen Reprogramming Kit is functionally validated for successful RNA-based reprogramming of adult fibroblasts, human umbilical vein endothelial cells (HUVECs), EPCs, and UPCs. Complete reprogramming of iPS cell colonies is confirmed by expression of pluripotency markers and appropriate colony morphology. All components of the kit are sterile and have tested negative for Mycoplasma spp. |
| Recommended Usage | For use with associated StemRNA 3rd Gen Reprogramming Kit protocols for generation of iPS cells. |
| Kit Contents | – OKSMNL NM-RNA (Part No. 05-0040), 30 µg, 1 vial |
RESOURCES
Specification Sheet
SS-STEMGENT-00-0076-D001002
Safety Data Sheets
SDS-STEMGENT-00-0076-D001002
SDS-STEMGENT-05-00xx-D001002-NM-RNA
Protocols
Protocol Stemgent StemRNA-3rd Gen Reprogramming Kit for Reprogramming Blood-Derived EPCs
Protocol Stemgent StemRNA 3rd Gen Reprogramming Kit for Reprogramming Adult and Neonatal Human Fibroblasts
Protocol Stemgent StemRNA 3rd Gen Reprogramming Kit for Reprogramming Urine-Derived Progenitor Cells (UPCs)
FAQ
Frequently Asked Questions REPROCELL Custom iPSC Services
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