Alkaline phosphatase (AP) is expressed at high levels in pluripotent stem cells. AP dephosphorylates nucleotides, proteins, and alkaloids under alkaline conditions. After staining, undifferentiated cells appear red or purple whereas differentiated cells appear colorless.
The undifferentiated state of embryonic stem (ES) and induced pluripotent stem (iPS) cells can be characterized by a high level of alkaline phosphatase (AP) expression which, along with the expression of other surface markers, indicates undifferentiated cells with the potential to self-renew. AP is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. When fixed ES or iPS cells are stained using the AP Staining Kit, undifferentiated cells appear red or purple, whereas differentiated cells appear colorless.
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